منابع مشابه
On the determination of xanthine oxidase activity in animal tissues.
Studies, undertaken in this laboratory to evaluate the effect, of riboflavin antagonists on mammalian flavoenzymes, have necessitated the investigation of the xanthine oxidase activity of mouse tissue. In general, little difficulty has been encountered in the manometric determination of xanthine oxidase (1) in tissues of high enzymatic activity, e.g. liver and small intestine, although irregula...
متن کاملIsolation of Different Animal Liver Xanthine Oxidase Containing Fractions and Determination of Kinetic Parameters for Xanthine
Xanthine oxidase activity containing fractions from rat, mouse, rabbit and guinea pig livers were obtained by heat treatment and ammonium sulfate precipitation. Xanthine oxidase activity was observed in rat and mouse liver fractions, while xanthine oxidase activity was absent in rabbit and guinea pig liver fractions. Enzyme kinetic parameters, K m and V max , were determined for the conversion ...
متن کاملXanthine oxidase: introductory remarks.
At the 661st Meeting of the Biochemical Society, held at Bath, the Host Colloquium, a joint meeting with the Society for Free Radical Research, took place on 10th and 11th April 1997. Its title was ‘Xanthine oxidase: enzymology and pathophysiology’. The two aspects of xanthine oxidase research specified in the Colloquium title have developed remarkably separately, and, for a number of reasons, ...
متن کاملHydroxylamine Inhibition of Xanthine Oxidase
In the course of investigations regarding the effects of various chemical agents on neoplast,ic purine metabolism, it was observed that hydroxylamine prevents the accumulation of uric acid or allantoin that normally occurs upon incubation of crude tissue homogenates. Subsequent studies, reported in this paper, demonstrate that hydroxylamine is a competitive inhibitor of xanthine oxidase activit...
متن کاملThe chemistry of xanthine oxidase. Electron-spin resonance of xanthine oxidase solutions.
The participation of metals in the action ofmetalloflavoproteins is the subject of much controversy, and it has recently been concluded by Singer & Massey (1957) that only for nitrate reductase has strong evidence been presented for valency changes in the metal in the course of the enzymic reactions. Studies on the function of the molybdenum, iron and flavinadenine dinucleotide of xanthine oxid...
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ژورنال
عنوان ژورنال: Pharmaceutica Analytica Acta
سال: 2013
ISSN: 2153-2435
DOI: 10.4172/2153-2435.s7-004